• MitMAB for Endocytosis Assays in Intestinal Organoids

  2026-08-22

  MitMAB enables polarity-aware analysis of dynamin-dependent uptake in intestinal organoids. This article translates milk-derived extracellular vesicle findings into a practical framework for causal endocytosis and intracellular trafficking research.

• Gentamycin Sulfate: From Ribosome to Resistance

  2026-08-22

  Gentamycin Sulfate is an aminoglycoside antibiotic for connecting ribosomal decoding errors with resistance phenotypes. This article presents an assay-design framework informed by comparative susceptibility testing in resistant Gram-negative pathogens.

• Sodium Ascorbate: From ROS to Assay Design

  2026-08-21

  Sodium Ascorbate is a mineral salt of ascorbic acid used to study oxidative-stress-driven cancer phenotypes. This article connects its ROS biology and glioblastoma evidence with biomarker-aware assay design while clarifying practical limitations and research-only use.

• Ruthenium Red for Calcium Mechanotransduction

  2026-08-20

  Use Ruthenium Red as a water-soluble Ca2+ transport inhibitor to test whether calcium flux contributes to compression-induced autophagy. This workflow connects cytoskeletal mechanotransduction with mitochondrial calcium uptake inhibition and provides practical controls for separating calcium effects from general toxicity or altered autophagy flux.

• Tofacitinib Repairs RA Macrophage Dysfunction

  2026-08-20

  A 2026 study identifies a GM-CSF-driven macrophage state in rheumatoid arthritis that couples inflammatory activation to oxidative stress and mitochondrial fragmentation. It shows that tofacitinib, unlike selected metabolic interventions, broadly restores regulatory, metabolic, and mitochondrial features through suppression of GM-CSFRα-associated STAT5 signaling.

• Paroxetine, Histone Serotonylation, and Melanoma Pyroptosis

  2026-08-19

  Li et al. identify paroxetine hydrochloride as a repurposing candidate that induces pyroptosis in BRAF V600E-mutated melanoma, including models resistant to BRAF and MEK inhibitors. The study links 5-HT reuptake blockade to reduced H3Q5 serotonylation at DNA-repair gene promoters, genomic instability, proteostasis stress, and enhanced anti-PD-1 activity in preclinical models.

• Chloroquine Diphosphate in Autophagy Assays

  2026-08-19

  Chloroquine Diphosphate offers a water-soluble way to interrogate late autophagy, treatment resistance, and cell-death crosstalk without introducing DMSO. This workflow-focused guide shows how to use SKU A8628 in dose-response studies, chemotherapy sensitization, radiotherapy sensitization, and mechanistic cancer research.

• BODIPY 581/591 C11: Reliable Lipid Assays

  2026-08-18

  Learn how BODIPY 581/591 C11 (SKU C8003) supports ratiometric lipid peroxidation detection in live-cell oxidative stress and ferroptosis workflows. This scenario-based guide covers assay design, protocol optimization, interpretation, controls, and practical product selection.

• ATS-9R: Designing Better Adipose Gene-Silencing Studies

  2026-08-18

  ATS-9R enables targeted nucleic acid delivery to white adipose tissue through Prohibitin-mediated uptake and nona-arginine condensation. This guide focuses on the assay-design decisions that connect particle formation, visceral adipose targeting, macrophage biology, and metabolic endpoints.

• BCKAs Activate HIF1α Signaling in Normoxia

  2026-08-17

  This preprint identifies branched-chain α-ketoacids (BCKAs) as paracrine metabolic signals that activate HIF1α in vascular cells despite ambient oxygen levels. Its findings connect BCKA metabolism, PHD2 inhibition, L2HG production, glycolytic remodeling, and pulmonary artery smooth muscle cell phenotypic switching, providing a framework for studying metabolic control of pulmonary vascular disease.

• Aprotinin Workflows for Protease Research

  2026-08-17

  Aprotinin provides reversible control of trypsin, plasmin, and kallikrein for mechanistic assays, fibrinolysis studies, and inflammation-focused workflows. This guide shows how to build concentration-response experiments, reduce handling variability, and interpret a complementary GRO-seq workflow without overstating cross-domain evidence.

• Localized BDNF Release Shapes NMJ Development

  2026-08-16

  This study identifies muscle-derived BDNF as a spatially regulated signal that supports the earliest stages of postsynaptic acetylcholine receptor organization at neuromuscular junctions. By combining live imaging, proteolytic perturbation, and a muscle-specific knockout model, it connects BDNF trafficking, localized calcium-dependent release, and precursor processing with neuromuscular synapse assembly.

• Dihydrotestosterone: Designing Better Androgen Assays

  2026-08-15

  Dihydrotestosterone is more than an androgen receptor agonist: it is a context-dependent perturbation tool for connecting receptor activity with EGFR, ERBB2, muscle, and prostate phenotypes. This guide shows how to design and interpret DHT experiments using evidence from cancer, BPH, and ALS models.

• Asymmetric Cu Single-Atom Nanozyme for AMI

  2026-08-14

  This study develops an asymmetrically coordinated Cu single-atom nanozyme, Cu-BrN3/SAN@M, to address oxidative and inflammatory injury after acute myocardial infarction. By combining Br-tuned Cu coordination with a biomimetic membrane interface, the material enhances reactive oxygen species clearance while remodeling macrophage and regulatory T-cell responses, offering a mechanistically integrated strategy for myocardial repair.

• Batimastat (BB-94) MMP Research Workflows

  2026-08-14

  Batimastat (BB-94) offers broad-spectrum control of zinc-dependent MMP activity for mechanistic studies spanning tumor invasion and neuromuscular synapse development. This guide translates its biochemical profile into practical assay workflows, dose-finding strategies, and troubleshooting checkpoints.

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